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Article

Method for introducing specific and unmarked mutations into the chromosome of Streptococcus pneumoniae.

Molecular biotechnology - 1 Jan 2004

Iannelli Francesco, Pozzi Gianni

Abstract excerpt

This work describes a procedure for the generation of site-specific mutations into the chromosome of Streptococcus pneumoniae that does not involve the use of an antibiotic resistance marker. A linear fragment of transforming deoxyribonucleic acid (DNA) is constructed by polymerase chain reaction (PCR) (gene splicing by overlap extension) and used to transform competent cells of S. pneumoniae. Selection of...

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PMID: 14734825
DOI: 10.1385/MB:26:1:81

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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