Article
High-efficiency transformation and gene inactivation in Streptococcus suis type 2.
Microbiology (Reading, England) - 1 Jan 1995
Smith H E, Wisselink H J, Vecht U, Gielkens A L, Smits M A
Abstract excerpt
An efficient electrotransformation system for Streptococcus suis type 2 is described. It is demonstrated that vectors based on the broad-host-range plasmid pWVO1 replicate in S. suis type 2. Transformation efficiencies of about 10(7) transformants per micrograms of plasmid DNA could be obtained. Derivatives of plasmid pBR322 containing S. suis chromosomal DNA did not replicate but integrated into the chromosome....
Topics
- Blotting, Southern
- Blotting, Western
- Chromosomes, Bacterial
- Crossing Over, Genetic
- DNA Replication
- DNA, Bacterial
- Electric Stimulation
- Enzyme-Linked Immunosorbent Assay
- Genes, Bacterial
- Mutation
- Plasmids