Article
Construction and analysis of a Streptococcus parasanguis recA mutant: homologous recombination is not required for adhesion in an in vitro tooth surface model.
Journal of bacteriology - 1 Jan 1999
Froeliger E H, Tomich M, Fives-Taylor P
Abstract excerpt
PCR was used to amplify an internal region of the recA gene from Streptococcus parasanguis FW213. The PCR fragment was used as a probe to recover the entire streptococcal recA gene from an S. parasanguis genomic library, and the sequence of the gene was determined. The deduced product of the S. p...
Topics
- Adhesins, Bacterial
- Bacterial Adhesion
- Base Sequence
- DNA Primers
- DNA, Bacterial
- Durapatite
- Gene Expression
- Genes, Bacterial
- Humans
- In Vitro Techniques
- Models, Biological
- Molecular Sequence Data
- Mutagenesis, Insertional
- Mutation
- Phenotype
- Rec A Recombinases
- Recombination, Genetic
- Restriction Mapping