p53 mutation in fresh lymphocytes, B-lymphoblastoid cell lines and their transformed cell lines originating from Bloom syndrome patients.

Abstract excerpt

To elucidate the molecular basis for malignant and premalignant states in Bloom syndrome (BS), p53 mutations were analyzed using Southern blot analysis and DNA sequencing of exons 5-9. p53 point mutations with and without loss of heterozygosity on 17p were detected in malignantly transformed BS c...

Topics

• Base Sequence
• Bloom Syndrome
• Blotting, Southern
• Cell Line
• Cell Line, Transformed
• Chromosome Deletion
• Genes, p53
• Lymphocytes
• Molecular Sequence Data
• Mutation
• Point Mutation
• Polymerase Chain Reaction