Article
Rapid and simple subtyping of the HLA-DRB3 gene in Graves' disease by using temperature-gradient gel electrophoresis.
Human immunology - 1 Mar 1993
Chen M, Maerz W, Manfras B J, Kuehnl P, Usadel K H, Boehm B O
Abstract excerpt
A HLA-DRB3-subtyping system that uses TGGE for analyzing DRB3 alleles was developed. The polymorphic second exon of the HLA-DRB3 gene was amplified from four homozygous typing cell lines, 223 healthy individuals, and 102 patients with Graves' disease by using the PCR. The PCR products were electr...
Topics
- Alleles
- Autoimmune Diseases
- Base Sequence
- DNA Probes, HLA
- DNA, Single-Stranded
- Disease Susceptibility
- Electrophoresis, Polyacrylamide Gel
- Genetic Predisposition to Disease
- Graves Disease
- HLA-DR Antigens
- HLA-DRB3 Chains