Article
Secretion of glycosylation site mutants can be rescued by the signal/pro sequence of tissue plasminogen activator.
Journal of cellular biochemistry - 1 Dec 1999
Köhne C, Johnson A, Tom S, Peers D H, Gehant R L, Hotaling T A, Brousseau D, Ryll T, Fox J A, Chamow S M, Berman P W
Abstract excerpt
Strategies that prevent the attachment of N-linked carbohydrates to nascent glycoproteins often impair intracellular transport and secretion. In the present study, we describe a method to rescue the intracellular transport and secretion of glycoproteins mutagenized to delete N-linked glycosylation sites. Site-directed mutagenesis was used to delete N-linked glycosylation sites from a chimeric protein, TNFR-IgG1....
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