HPLC method for rapid determination of acetylator phenotype by measuring urinary caffeine metabolites.

doi:10.1016/0731-7085(93)80063-7

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Article

HPLC method for rapid determination of acetylator phenotype by measuring urinary caffeine metabolites.

Journal of pharmaceutical and biomedical analysis - 1 Oct 1993

Klebovich I, Arvela P, Pelkonen O

Abstract excerpt

A validated reversed-phase high-performance liquid chromatographic (RP-HPLC) method is developed for the selective and rapid determination of two major metabolites of caffeine, namely 5-acetylamino-6-formylamino-3-methyluracil (AFMU) and 1-methylxanthine (MX) from human urine. HPLC separation is...

Topics

Caffeine
Chromatography, High Pressure Liquid
Humans
Phenotype
Reproducibility of Results
Uracil
Xanthines

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