Determination of N-acetylator phenotype using caffeine as a probe compound: a comparison of high-performance liquid chromatography and capillary electrophoresis methods.

Abstract excerpt

A test for determining N-acetylator metabolic phenotype has been developed using caffeine as a probe drug. A spot sample of urine is taken, and the unextracted urine is then analysed by micellar electrokinetic capillary chromatography. Phenotype is determined from the peak-area ratio of urinary 5-acetylamino-6-formylamino-3-methyluracil to 1-methylxanthine. Phenotype assignments using this method were compared...

Topics

• Acetylation
• Caffeine
• Chromatography, High Pressure Liquid
• Electrophoresis
• Humans
• Phenotype
• Reference Values
• Reproducibility of Results