Zinc finger phage: affinity selection of fingers with new DNA-binding specificities.

doi:10.1126/science.8303274

Back to search

Article

Zinc finger phage: affinity selection of fingers with new DNA-binding specificities.

Science (New York, N.Y.) - 4 Feb 1994

Rebar E J, Pabo C O

Abstract excerpt

A phage display system was developed and used to select zinc finger proteins with altered DNA-binding specificities. The three zinc fingers of the Zif268 protein were expressed on the surface of filamentous phage, and a library of variants was prepared by randomizing critical amino acids in the f...

Topics

Amino Acid Sequence
Bacteriophages
Base Sequence
Cloning, Molecular
DNA
DNA-Binding Proteins
Genetic Variation
Genetic Vectors
Molecular Sequence Data
Protein Binding
Transcription Factors
Zinc Fingers

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.