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Article

A mutagenesis system utilizing a Tn1722 derivative containing an Escherichia coli-specific vector plasmid: application to Pseudomonas species.

Gene - 22 Dec 1993

Tsuda M, Nakazawa T

Abstract excerpt

A novel transposon (Tn) mutagenesis system for Gram- non-enteric bacteria was developed which allowed rapid and one-step cloning of the mutated region in Escherichia coli. The Tn constructed was Tn1722-299Km, a Tn1722 derivative containing a KmR gene and the entire sequence of an E. coli-specific plasmid, pACYC184. The hybrid plasmid consisting of Tn1722-299Km and the transfer genes of plasmid R388 was conjugally...

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Identifiers

PMID: 8294012
DOI: 10.1016/0378-1119(93)90475-i

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