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Article

Transposon mutagenesis of Clostridium acetobutylicum P262: isolation and characterization of solvent deficient and metronidazole resistant mutants.

FEMS microbiology letters - 15 Dec 1993

Babb B L, Collett H J, Reid S J, Woods D R

Abstract excerpt

An efficient transposon mutagenesis system using conjugative transposons Tn916 and Tn925::Tn917 was established for Clostridium acetobutylicum P262, an industrial strain which has proved difficult to manipulate genetically. Transposon insertions occurred at several different locations to produce a variety of mutants. An oligosporogenous mutant deficient in acetone and butanol production, and two...

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PMID: 8288111
DOI: 10.1111/j.1574-6968.1993.tb06596.x

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