Article
Effects of lysine-sensitive aspartokinase III on lysine biosynthesis in Escherichia coli K-12.
Proceedings of the National Science Council, Republic of China. Part B, Life sciences - 1 Jul 1993
Huang K J, Hseu T H
Abstract excerpt
A promoterless lysC gene, coding for Escherichia coli aspartokinase III (AKase III), has been cloned by phenotypic complementation using plasmid pUC19 as the vector. The hybrid plasmid obtained, pUC19AK3, preserved the ribosome binding site and transcriptional termination signal of the gene but with a lac promoter. E. coli strains containing the recombinant plasmid had high levels of AKase III activity. AKase III...
Topics
- Amino Acid Sequence
- Aspartate Kinase
- Aspartic Acid
- Base Sequence
- Chromatography, High Pressure Liquid
- Cloning, Molecular
- Culture Media
- DNA, Bacterial
- Escherichia coli
- Gene Expression
- Lysine
