Article
Partial characterization of a lysU mutant of Escherichia coli K-12.
Journal of bacteriology - 1 Mar 1991
Hassani M, Saluta M V, Bennett G N, Hirshfield I N
Abstract excerpt
The Escherichia coli K-12 strain GNB10181 shows no inducible lysyl-tRNA synthetase (LysRS) activity. Two-dimensional gel electrophoretic analysis of the polypeptides synthesized by this strain indicates that the normal lysU gene product, LysU, is absent. When both GNB10181 and its parent, MC4100,...
Topics
- Cloning, Molecular
- Electrophoresis, Gel, Two-Dimensional
- Enzyme Induction
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Genetic Complementation Test
- Heat-Shock Proteins
- Hot Temperature
- Lysine-tRNA Ligase
- Mutation
