Article
Xis protein of the conjugative transposon Tn916 plays dual opposing roles in transposon excision.
Molecular microbiology - 1 Sept 2001
Hinerfeld D, Churchward G
Abstract excerpt
The binding of Tn916 Xis protein to its specific sites at the left and right ends of the transposon was compared using gel mobility shift assays. Xis formed two complexes with different electrophoretic mobilities with both right and left transposon ends. Complex II, with a reduced mobility, formed at higher concentrations of Xis and appeared at an eightfold lower Xis concentration with a DNA fragment from the...
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