Article
Attenuation of a reactivated cardiovirulent coxsackievirus B3: The 5'-nontranslated region does not contain major attenuation determinants.
Journal of medical virology - 1 Oct 1993
Zhang H Y, Yousef G E, Cunningham L, Blake N W, OuYang X, Bayston T A, Kandolf R, Archard L C
Abstract excerpt
To investigate the molecular basis of pathogenicity of Coxsackieviruses, a virus was reactivated by transfection from a full-length cDNA clone derived from cardiovirulent Coxsackievirus B3 (CVB3). The reactivated virus, rCVB3, was passaged serially in human dermatofibroblasts (HDF). No cytopathic...
Topics
- Animals
- Base Sequence
- Cells, Cultured
- DNA, Viral
- Disease Models, Animal
- Enterovirus B, Human
- Fibroblasts
- Humans
- Introns
- Male
- Molecular Sequence Data
- Myocarditis
- Phenotype
- Polymerase Chain Reaction
- Transfection
- Vero Cells
- Virulence