Article
Tracing B cell development in human germinal centres by molecular analysis of single cells picked from histological sections.
The EMBO journal - 15 Dec 1993
Küppers R, Zhao M, Hansmann M L, Rajewsky K
Abstract excerpt
Germinal centres are areas of intense B lymphocyte proliferation inside primary B cell follicles in spleen and lymph nodes. Rearranged V genes from single human B cells, isolated from histological sections of two such structures by micromanipulation, were amplified and sequenced. Cells from the f...
Topics
- Adult
- Amino Acid Sequence
- B-Lymphocytes
- Base Sequence
- Cell Differentiation
- Child
- DNA Primers
- Gene Rearrangement, B-Lymphocyte
- Genes, Immunoglobulin
- Humans
- Immunoglobulin Heavy Chains
- Immunoglobulin Variable Region
- Lymph Nodes
- Micromanipulation
- Molecular Sequence Data
- Mutation
- Sequence Alignment
- Sequence Homology, Nucleic Acid