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Article

PCR detection of cytokines in normal human and pagetic osteoblast-like cells.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research - 1 Oct 1993

Birch M A, Ginty A F, Walsh C A, Fraser W D, Gallagher J A, Bilbe G

Abstract excerpt

We investigated the expression of cytokine transcripts in osteoblast-like cells derived from explants of pagetic and normal bone. A reverse transcription-linked PCR was used that allowed the simultaneous analysis of a range of cytokines. Normal osteoblast-like cells were found to contain the transcripts for IL-1 beta, IL-6, and TGF-beta 1. For the first time we detected in bone cells the two other mammalian...

Topics

Base Sequence
Cells, Cultured
Cytokines
DNA, Complementary
Humans
Interleukins
Molecular Sequence Data
Osteitis Deformans
Osteoblasts
Phenotype
Polymerase Chain Reaction

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

RNA, Messenger

Transcription, Genetic

Transforming Growth Factors

Tumor Cells, Cultured

Source

Identifiers

PMID: 8256652
DOI: 10.1002/jbmr.5650081002

Genes

TNF

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8256652-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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