Article
The effect of interleukin-1 on cytokine gene expression in cultured human articular chondrocytes analyzed by messenger RNA phenotyping.
Arthritis and rheumatism - 1 Jan 1993
Seid J M, Rahman S, Graveley R, Bunning R A, Nordmann R, Wishart W, Russell R G
Abstract excerpt
OBJECTIVE: To investigate the pattern of cytokine gene expression in human articular chondrocytes in culture in response to interleukin-1 beta (IL-1 beta). The effect of serum and variations in culture conditions was also studied. METHODS: Messenger RNA was extracted from cells, reverse-transcribed to complementary DNA, and amplified by the polymerase chain reaction (PCR), using specific oligonucleotide primers....
Topics
- Base Sequence
- Blotting, Southern
- Cartilage, Articular
- Cells, Cultured
- Cytokines
- Electrophoresis, Agar Gel
- Gene Expression
- Humans
- Interleukin-1
- Molecular Sequence Data
- Phenotype