Cytokine mRNA and protein expression by cell cultures of epithelial ovarian cancer—Methodological considerations on the choice of analytical method for cytokine analyses

Abstract excerpt

PROBLEM: To get a comprehensive picture of cytokine expression in health and disease is difficult, cytokines are transiently and locally expressed, and protein analyses are burdened by biological modifications, technical issues, and sensitivity to handling of samples. Thus, alternative methods, based on molecular techniques for cytokine mRNA analyses, are often used. We compared cytokine mRNA and protein...

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