Genetic methods for study of trans-acting genes involved in processing of precursors to yeast cytoplasmic transfer RNAs.

doi:10.1016/0076-6879(90)81139-l

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Article

Genetic methods for study of trans-acting genes involved in processing of precursors to yeast cytoplasmic transfer RNAs.

Methods in enzymology - 1 Jan 1990

Hopper A K

Abstract excerpt

All of the strategies to identify mutants defective in pre-tRNA processing have proved to be useful. However, in some of these strategies (such as inability to derepress a nonsense suppressor, correction of processing-defective substrates, and reverse genetics) only a single mutant has been isola...

Topics

Alleles
Blotting, Northern
Cytoplasm
Genes, Fungal
Genetic Techniques
Genotype
Mutation
RNA Precursors
RNA Processing, Post-Transcriptional
RNA, Fungal
RNA, Transfer
Radioisotope Dilution Technique
Saccharomyces cerevisiae
Suppression, Genetic
Tritium
Uracil

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