Article
Genetic methods for study of trans-acting genes involved in processing of precursors to yeast cytoplasmic transfer RNAs.
Methods in enzymology - 1 Jan 1990
Hopper A K
Abstract excerpt
All of the strategies to identify mutants defective in pre-tRNA processing have proved to be useful. However, in some of these strategies (such as inability to derepress a nonsense suppressor, correction of processing-defective substrates, and reverse genetics) only a single mutant has been isolated. This is not because the methods have proved to be inefficient, but rather because the field is still in infancy....
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