Article
Cells with a familial Alzheimer's disease mutation produce authentic beta-peptide.
Neuroreport - 1 Aug 1993
Dovey H F, Suomensaari-Chrysler S, Lieberburg I, Sinha S, Keim P S
Abstract excerpt
Cells overexpressing the beta-amyloid precursor protein possessing a mutation found in familial Alzheimer's disease overproduce beta-amyloid peptide (A beta). Because these findings were based on immunological identification, we have chemically characterized the peptides produced. Purified A beta fragments from the conditioned media of these cells were found to have N-terminal sequence consistent with the A beta...
Topics
- Alzheimer Disease
- Amino Acid Sequence
- Amyloid beta-Peptides
- Cell Line
- Chromatography
- Enzyme-Linked Immunosorbent Assay
- Humans
- Immunologic Techniques
- Mass Spectrometry
- Molecular Sequence Data
- Mutation