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Article

Genomic DNA functions as a universal external standard in quantitative real-time PCR.

Nucleic acids research - 13 Jul 2006

Yun James J, Heisler Lawrence E, Hwang Irene I L, Wilkins Olivia, Lau Suzanne K, Hyrcza Martin, Jayabalasingham Bamini, Jin Jing, McLaurin JoAnne, Tsao Ming-Sound, Der Sandy D

Abstract excerpt

Real-time quantitative PCR (qPCR) is a powerful tool for quantifying specific DNA target sequences. Although determination of relative quantity is widely accepted as a reliable means of measuring differences between samples, there are advantages to being able to determine the absolute copy numbers of a given target. One approach to absolute quantification relies on construction of an accurate standard curve using...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 16840529
DOI: 10.1093/nar/gkl400
PMCID: PMC1524913

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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