Article
Real-Time PCR for Gene Expression Quantification in Asthma.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2016
Segundo-Val Ignacio San, García-Solaesa Virginia, García-Sánchez Asunción
Abstract excerpt
The quantitative real-time PCR (qPCR) has become the reference technique for studying gene expression in recent years. The application of qPCR to the study of asthma provides very useful information regarding the gene expression mechanisms. The quantification of RNA from cDNA can be performed by using fluorescent dyes or specific sequence probes. Here, we describe the protocol to quantify gene expression levels...
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