Article
Roulette mutagenesis of the FMN-binding site of Klebsiella pneumoniae flavodoxin.
European journal of biochemistry - 1 Oct 1993
Drummond M, Huff S, Green A
Abstract excerpt
A method of randomising specific regions of coding sequences has been devised which utilises the Lac phenotype to identify mutants. Intact genes can be mutagenised, making it unnecessary to reclone the mutations before examining mutant phenotypes. The method has been applied to three residues around the N-terminus of the first alpha helix of the Klebsiella pneumoniae nitrogenase flavodoxin, which are predicted to...
Topics
- Amino Acid Sequence
- Base Sequence
- Binding Sites
- Codon
- Flavin Mononucleotide
- Flavodoxin
- Glycine
- Klebsiella pneumoniae
- Lysine
- Molecular Sequence Data
- Mutagenesis
- Nitrogenase