Article
In vitro genetic analysis of the structural features of the pre-tRNA required for determination of the 3' splice site in the intron excision reaction.
The EMBO journal - 1 Dec 1993
Bufardeci E, Fabbri S, Baldi M I, Mattoccia E, Tocchini-Valentini G P
Abstract excerpt
During processing of intron-containing pre-tRNAs, the Xenopus laevis splicing endonuclease binds the precursor and cleaves it at both the 5' and 3' splice sites. In vitro selection was used to determine structural features characteristic of precursor tRNA molecules that are active in this reactio...
Topics
- Animals
- Anticodon
- Base Composition
- Base Sequence
- DNA
- Endonucleases
- Introns
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- RNA Precursors
- RNA Splicing
- RNA, Transfer, Phe
- Xenopus laevis