Article
Participation of the intron in the reaction catalyzed by the Xenopus tRNA splicing endonuclease.
Science (New York, N.Y.) - 13 Mar 1992
Baldi M I, Mattoccia E, Bufardeci E, Fabbri S, Tocchini-Valentini G P
Abstract excerpt
Introns have generally been assumed to be passive in the transfer RNA splicing reaction. Experiments have now been done showing that the endonuclease is able to cut a precursor provided that a base in the single-stranded loop of the intron can pair with the base of the 5' exon situated at the position that immediately follows the anticodon stem (position 33 in the yeast tRNA isoacceptor pre-tRNA(Leu)3, position...
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