Article
Cleavage of pre-tRNAs by the splicing endonuclease requires a composite active site.
Nature - 18 May 2006
Trotta Christopher R, Paushkin Sergey V, Patel Meenal, Li Hong, Peltz Stuart W
Abstract excerpt
Splicing is required for the removal of introns from a subset of transfer RNAs in all eukaryotic organisms. The first step of splicing, intron recognition and cleavage, is performed by the tRNA-splicing endonuclease, a tetrameric enzyme composed of the protein subunits Sen54, Sen2, Sen34 and Sen15. It has previously been demonstrated that the active sites for cleavage at the 5' and 3' splice sites of precursor...
Topics
- Archaea
- Binding Sites
- Cations
- Endoribonucleases
- Eukaryotic Cells
- Models, Biological
- Mutation
- RNA Precursors
- RNA Splice Sites
- RNA Splicing
- RNA, Transfer
- RNA, Transfer, Phe
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins