Domain structure of phage P4 alpha protein deduced by mutational analysis.

doi:10.1128/jb.177.15.4333-4341.1995

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Article

Domain structure of phage P4 alpha protein deduced by mutational analysis.

Journal of bacteriology - 1 Aug 1995

Ziegelin G, Linderoth N A, Calendar R, Lanka E

Abstract excerpt

Bacteriophage P4 DNA replication depends on the product of the alpha gene, which has origin recognition ability, DNA helicase activity, and DNA primase activity. One temperature-sensitive and four amber mutations that eliminate DNA replication in vivo were sequenced and located in the alpha gene. Sequence analysis of the entire gene predicted a domain structure for the alpha polypeptide chain (777 amino acid...

Topics

Amino Acid Sequence
Base Sequence
Coliphages
DNA Helicases
DNA Primase
DNA Replication
DNA, Viral
DNA-Binding Proteins
Gene Expression Regulation, Viral
Molecular Sequence Data
Mutagenesis, Site-Directed

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Myoviridae

Prokaryotic Cells

RNA Nucleotidyltransferases

Replication Origin

Sequence Homology, Amino Acid

Transcriptional Activation

Source

Open in PubMed Open full text

Identifiers

PMID: 7635818
DOI: 10.1128/jb.177.15.4333-4341.1995
PMCID: PMC177181

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7635818-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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