Article
A method for accurate amplification of polymorphic CA-repeat sequences.
PCR methods and applications - 1 Aug 1993
Odelberg S J, White R
Abstract excerpt
Anomalous PCR products are often produced during the amplification of d(CA)n.d(TG)n sequences. Upon denaturing polyacrylamide gel electrophoresis, these products yield a ladder-like pattern that can complicate genotypic interpretation. We have developed two related techniques, referred to as two- and three-stage linear amplification (2-SLA and 3-SLA, respectively), which largely overcome this problem and yield...
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