A method for accurate amplification of polymorphic CA-repeat sequences.

doi:10.1101/gr.3.1.7

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Article

A method for accurate amplification of polymorphic CA-repeat sequences.

PCR methods and applications - 1 Aug 1993

Odelberg S J, White R

Abstract excerpt

Anomalous PCR products are often produced during the amplification of d(CA)n.d(TG)n sequences. Upon denaturing polyacrylamide gel electrophoresis, these products yield a ladder-like pattern that can complicate genotypic interpretation. We have developed two related techniques, referred to as two-...

Topics

Adenine
Alleles
Base Sequence
Cytosine
DNA
DNA Primers
Electrophoresis, Polyacrylamide Gel
Female
Genetic Markers
Genotype
Humans
Male
Molecular Sequence Data
Pedigree
Polymerase Chain Reaction
Polymorphism, Genetic
Repetitive Sequences, Nucleic Acid

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