Article
High-throughput AFLP analysis using infrared dye-labeled primers and an automated DNA sequencer.
BioTechniques - 1 Feb 2001
Myburg A A, Remington D L, O'Malley D M, Sederoff R R, Whetten R W
Abstract excerpt
Amplified fragment length polymorphism (AFLP) analysis is currently the most powerful and efficient technique for the generation of large numbers of anonymous DNA markers in plant and animal genomes. We have developed a protocol for high-throughput AFLP analysis that allows up to 70,000 polymorphic marker genotype determinations per week on a single automated DNA sequencer. This throughput is based on multiplexed...
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