Article
PCR amplification of specific alleles (PASA) is a general method for rapidly detecting known single-base changes.
BioTechniques - 1 Jan 1992
Sommer S S, Groszbach A R, Bottema C D
Abstract excerpt
Previous data suggested that PCR amplification of specific alleles (also known as allele-specific amplification and amplification refractory mutation system) is not a general method for rapidly and accurately detecting known single-base changes. Herein we present our experience with the use of PC...
Topics
- Alleles
- Base Sequence
- DNA
- DNA-Directed DNA Polymerase
- Humans
- Magnesium
- Molecular Sequence Data
- Mutation
- Oligonucleotides
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Taq Polymerase
- Temperature
- Templates, Genetic