Large DNA fragment sizing using native acrylamide gels on an automated DNA sequencer and GENESCAN software.

Article

Large DNA fragment sizing using native acrylamide gels on an automated DNA sequencer and GENESCAN software.

BioTechniques - 1 Sept 1998

McEvoy C R, Seshadri R, Firgaira F A

Abstract excerpt

We have investigated the potential of the PE Applied Biosystems Model 373 Automated DNA Sequencer and GENESCAN software to size minisatellite alleles ranging in size from 230 bp to 2.5 kbp. We report on the use of a native (non-denaturing) acrylamide gel system and fluorescent dUTP labeling of PC...

Topics

Alleles
DNA
DNA Primers
Electrophoresis, Polyacrylamide Gel
Fluorescent Dyes
Genes, ras
Humans
Microsatellite Repeats
Polymerase Chain Reaction
Reproducibility of Results
Sequence Analysis, DNA
Software

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