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Large DNA fragment sizing using native acrylamide gels on an automated DNA sequencer and GENESCAN software.

BioTechniques - 1 Sept 1998

McEvoy C R, Seshadri R, Firgaira F A

Abstract excerpt

We have investigated the potential of the PE Applied Biosystems Model 373 Automated DNA Sequencer and GENESCAN software to size minisatellite alleles ranging in size from 230 bp to 2.5 kbp. We report on the use of a native (non-denaturing) acrylamide gel system and fluorescent dUTP labeling of PCR products. The observed variability in size calling ranged from +/- 0.4-bp standard deviation (SD) at the lower end of...

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PMID: 9762444

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