Formation of the meta II photointermediate is accompanied by conformational changes in the cytoplasmic surface of rhodopsin.

doi:10.1021/bi00096a012

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Article

Formation of the meta II photointermediate is accompanied by conformational changes in the cytoplasmic surface of rhodopsin.

Biochemistry - 16 Nov 1993

Resek J F, Farahbakhsh Z T, Hubbell W L, Khorana H G

Abstract excerpt

Five mutations of rhodopsin have been produced, each of which contains a unique cysteine residue at positions 62, 65, 140, 240, or 316 in the cytoplasmic domain. The single reactive cysteines were derivatized with a sulfhydryl-specific nitroxide spin-label, and the electron paramagnetic resonance...

Topics

Amino Acid Sequence
Animals
Blotting, Western
Cattle
Cysteine
Cytoplasm
Digitonin
Electron Spin Resonance Spectroscopy
Glucosides
Molecular Sequence Data
Mutation
Photochemistry
Protein Structure, Secondary
Rhodopsin

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