Article
Highly conserved tyrosine stabilizes the active state of rhodopsin.
Proceedings of the National Academy of Sciences of the United States of America - 16 Nov 2010
Goncalves Joseph A, South Kieron, Ahuja Shivani, Zaitseva Ekaterina, Opefi Chikwado A, Eilers Markus, Vogel Reiner, Reeves Philip J, Smith Steven O
Abstract excerpt
Light-induced isomerization of the 11-cis-retinal chromophore in the visual pigment rhodopsin triggers displacement of the second extracellular loop (EL2) and motion of transmembrane helices H5, H6, and H7 leading to the active intermediate metarhodopsin II (Meta II). We describe solid-state NMR measurements of rhodopsin and Meta II that target the molecular contacts in the region of the ionic lock involving...
Topics
- Alanine
- Amino Acid Substitution
- Animals
- Cattle
- Conserved Sequence
- Crystallography, X-Ray
- HEK293 Cells
- Humans
- Ion Channel Gating
- Magnetic Resonance Spectroscopy
- Mutant Proteins
- Mutation
- Protein Conformation