Article
Time-resolved detection of transient movement of helices F and G in doubly spin-labeled bacteriorhodopsin.
Biophysical journal - 1 Jun 2001
Radzwill N, Gerwert K, Steinhoff H J
Abstract excerpt
Photo-excited structural changes of the light-driven proton pump bacteriorhodopsin were monitored using double-site-directed spin labeling combined with electron paramagnetic resonance (EPR) spectroscopy. The inter-spin distances between nitroxides attached at residue positions 100 and 226, 101 and 160, and 101 and 168 were determined for the BR initial state and the trapped M photo-intermediate. Distance changes...
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