Characterization of herpes simplex virus type 1 thymidine kinase mutants engineered for improved ganciclovir or acyclovir activity.

doi:10.1110/ps.2460102

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Article

Characterization of herpes simplex virus type 1 thymidine kinase mutants engineered for improved ganciclovir or acyclovir activity.

Protein science : a publication of the Protein Society - 1 Sept 2002

Kokoris Mark S, Black Margaret E

Abstract excerpt

Herpes Simplex Virus type 1 (HSV-1) thymidine kinase (TK) is currently the most widely used suicide agent for gene therapy of cancer. Tumor cells that express HSV-1 thymidine kinase are rendered sensitive to prodrugs due to preferential phosphorylation by this enzyme. Although ganciclovir (GCV) is the prodrug of choice for use with TK, this approach is limited in part by the toxicity of this prodrug. From a...

Topics

Acyclovir
Amino Acid Sequence
Antineoplastic Agents
Antiviral Agents
Binding Sites
Chromatography, Affinity
Ganciclovir
Genetic Therapy
Herpesvirus 1, Human
Humans
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Structure

Mutation

Prodrugs

Substrate Specificity

Thymidine Kinase

Tumor Cells, Cultured

Source

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Identifiers

PMID: 12192082
DOI: 10.1110/ps.2460102
PMCID: PMC2373606

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