Article
The stimulation of pp42mapkinase by insulin does not correlate with its metabolic actions in cells overexpressing mutant insulin receptors.
Biochemical and biophysical research communications - 15 Oct 1993
Pang L, Lazar D F, Moller D E, Flier J S, Saltiel A R
Abstract excerpt
Naturally occurring human insulin receptor mutants Ser1200 and Thr1134, and a site-directed mutant Arg1030 overexpressed in Chinese hamster ovary (CHO) cells, bind insulin with affinities identical to wildtype receptors but are apparently kinase deficient. Cells expressing the Ser1200 receptor ex...
Topics
- Animals
- CHO Cells
- Calcium-Calmodulin-Dependent Protein Kinases
- Cricetinae
- Dose-Response Relationship, Drug
- Enzyme Activation
- Humans
- Insulin
- Mutation
- Phosphorylation
- Receptor, Insulin
- Recombinant Proteins
- Tyrosine