Article
Use of site-directed mutagenesis of allele-specific PCR primers to identify the GSTM1 A, GSTM1 B, GSTM1 A,B and GSTM1 null polymorphisms at the glutathione S-transferase, GSTM1 locus.
The Biochemical journal - 1 Oct 1993
Fryer A A, Zhao L, Alldersea J, Pearson W R, Strange R C
Abstract excerpt
We describe the identification of the GSTM1 null, GSTM1 A, GSTM1 B and GSTM1 A,B polymorphisms at the glutathione S-transferase GSTM1 locus using a single-step PCR method. Target DNA was amplified using primers to intron 6 and exon 7 with site-directed mutagenesis being used to introduce a restri...
Topics
- Alleles
- Base Sequence
- DNA Primers
- Glutathione Transferase
- Humans
- Liver
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Phenotype
- Polymerase Chain Reaction
- Polymorphism, Genetic