Mapping critical residues in eukaryotic DNA-binding proteins: a plasmid-based genetic selection strategy with application to the Oct-2 POU motif.

doi:10.1021/bi00186a017

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Article

Mapping critical residues in eukaryotic DNA-binding proteins: a plasmid-based genetic selection strategy with application to the Oct-2 POU motif.

Biochemistry - 24 May 1994

Botfield M C, Jancso A, Weiss M A

Abstract excerpt

Discrimination between allowed and disallowed amino acid substitutions provides a powerful method for analysis of protein structure and function. Site-directed mutagenesis allows specific hypotheses to be tested, but its systematic application to entire structural motifs is inefficient. This limi...

Topics

Base Sequence
Binding Sites
DNA
DNA-Binding Proteins
Escherichia coli
Gene Transfer Techniques
Genes, Reporter
Humans
Hydrogen Bonding
Lac Operon
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Mutagenesis, Site-Directed
Octamer Transcription Factor-2
Phenotype
Plasmids
Protein Structure, Secondary
Signal Recognition Particle

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Open in PubMed

Identifiers

PMID: 8193131
DOI: 10.1021/bi00186a017

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8193131-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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