Purification of viruses and macromolecular assemblies for structural investigations using a novel ion exchange method.

doi:10.1006/viro.1994.1259

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Article

Purification of viruses and macromolecular assemblies for structural investigations using a novel ion exchange method.

Virology - 15 May 1994

Walin L, Tuma R, Thomas G J, Bamford D H

Abstract excerpt

We describe a novel ion exchange chromatographic technique suitable for large-scale preparation of viruses and other biomacromolecular assemblies in highly purified form. The method, which utilizes anion exchange on either of two commercially available cellulose cartridges, is applied to the Esch...

Topics

Centrifugation, Zonal
Chromatography, DEAE-Cellulose
Chromatography, Ion Exchange
Mutation
Salmonella Phages
Spectrum Analysis, Raman
Viral Proteins
Virion
Viruses

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