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Mutational analysis of residues in the nucleotide binding domain of human terminal deoxynucleotidyl transferase.

1 Apr 1994

Abstract excerpt

Human terminal deoxynucleotidyl transferase (TdT) was overexpressed in a baculovirus system. The pure recombinant enzyme was identical in size, activity, kinetic constants, and metal effects to native enzyme. Three amino acids, within either the putative nucleotide binding domain and part of a DNA polymerase consensus sequence, YGDTDSLF, or a TdT consensus sequence, GGFRRGK, were altered by site-directed...

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PMID: 8163485
DOI: 10.1016/s0021-9258(17)32652-2

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