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Article

The key role of E418 carboxyl group in the formation of Nt.BspD6I nickase active site: Structural and functional properties of Nt.BspD6I E418A mutant.

Journal of structural biology - 1 Jun 2020

Artyukh Rimma I, Kachalova Galina S, Yunusova Alfiya K, Fatkhullin Bulat F, Atanasov Boris P, Perevyazova Tatyana A, Popov Alexander N, Gabdulkhakov Azat G, Zheleznaya Ludmila A

Abstract excerpt

The mutated nickase Nt.BspD6I E418A has been obtained by site-directed mutagenesis. The purified protein has been crystallized, and its spatial structure has been determined at 2.45 Å resolution. An analysis of the crystal structures of the wild-type and mutated nickase have shown that the elimination of a carboxyl group due to the E418A mutation initiates marked conformational changes in both the N-terminal...

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PMID: 32298813
DOI: 10.1016/j.jsb.2020.107508

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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