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Article

Transformation of mycobacterial species using hygromycin resistance as selectable marker

1 Jan 1994

Abstract excerpt

Electroporation with shuttle plasmids carrying a kanamycin resistance gene as a selectable marker failed to generate transformants in two mycobacterial species currently being used in human vaccine trials (Mycobacterium w and Mycobacterium vaccae). In contrast, efficient transformation [10(3)-10(5) transformants (micrograms DNA)-1] was obtained using novel vectors with selection based on expression of resistance...

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PMID: 8162182
DOI: 10.1099/13500872-140-1-133

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