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Article

[Optimizing Mycobacterium Recombineering System (pJV53) to Promote the Screening of the Mycobacterium Mutants].

Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition - 1 Sept 2019

Wang Chuhan, Ma Peng-Jiao, Luo Tao, Bao Lang

Abstract excerpt

OBJECTIVE: To establish a way for screening Mycobacterium mutants through adding the screening markers into pJV53. METHODS: The sucrose counter selection gene SacB and mutant hygromycin-resistant gene hygS were inserted into pJV53; The recovery of the hygromycin-resistance indicated the successful homologous recombination in Mycobacterium smegmatis (Ms), which could serve as mutant screening marker; The sucrose...

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PMID: 31762229

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