Article
Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis.
Molecular microbiology - 1 Nov 1990
Snapper S B, Melton R E, Mustafa S, Kieser T, Jacobs W R
Abstract excerpt
Recent development of vectors and methodologies to introduce recombinant DNA into members of the genus Mycobacterium has provided new approaches for investigating these important bacteria. While most pathogenic mycobacteria are slow-growing, Mycobacterium smegmatis is a fast-growing, non-pathogenic species that has been used for many years as a host for mycobacteriophage propagation and, recently, as a host for...
Topics
- DNA, Bacterial
- Escherichia coli
- Mutagenesis
- Mycobacterium
- Phenotype
- Plasmids
- Restriction Mapping
- Transformation, Bacterial