Article
Cell cycle regulation of the Escherichia coli nrd operon: requirement for a cis-acting upstream AT-rich sequence.
Journal of bacteriology - 1 Apr 1994
Sun L, Jacobson B A, Dien B S, Srienc F, Fuchs J A
Abstract excerpt
The expression of the nrd operon encoding ribonucleotide reductase in Escherichia coli has been shown to be cell cycle regulated. To identify the cis-acting elements required for the cell cycle regulation of the nrd promoter, different 5' deletions as well as site-directed mutations were translationally fused to a lacZ reporter gene. The expression of beta-galactosidase from these nrd-lacZ fusions in single-copy...
Topics
- Base Sequence
- Cell Cycle
- Cloning, Molecular
- Escherichia coli
- Flow Cytometry
- Gene Expression Regulation, Bacterial
- Gene Expression Regulation, Enzymologic
- Genes, Bacterial
- Molecular Sequence Data
- Mutation