Introduction of wild-type p53 in a human ovarian cancer cell line not expressing endogenous p53.

doi:10.1093/nar/22.6.1012

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Article

Introduction of wild-type p53 in a human ovarian cancer cell line not expressing endogenous p53.

Nucleic acids research - 25 Mar 1994

Vikhanskaya F, Erba E, D'Incalci M, Broggini M

Abstract excerpt

Utilizing a temperature sensitive p53 mutant (pLTRp53cGval135) which expresses mutant p53 at 37 degrees C and a wild-type like p53 at 32 degrees C, we transfected a human ovarian cancer cell line (SKOV3) which does not express endogenous p53. Among the different clones obtained, we selected three...

Topics

Animals
Apoptosis
Cell Division
Drug Resistance
Female
Flow Cytometry
Fluorescent Antibody Technique
Genes, p53
Humans
Mice
Mutation
Neomycin
Ovarian Neoplasms
Plasmids
RNA, Messenger
Temperature
Transfection
Tumor Cells, Cultured

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