Effect of traY amber mutations on F-plasmid traY promoter activity in vivo.

doi:10.1128/jb.178.19.5787-5789.1996

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Article

Effect of traY amber mutations on F-plasmid traY promoter activity in vivo.

Journal of bacteriology - 1 Oct 1996

Silverman P M, Sholl A

Abstract excerpt

We have examined the effect of the F plasmid TraY protein on tra gene expression in vivo. Expression was assayed as alkaline phosphatase activity in cells containing a traY phi(traA'-'phoA)hyb operon under traY promoter control. Amber mutations in traY significantly reduced alkaline phosphatase a...

Topics

Alkaline Phosphatase
Bacterial Proteins
Codon, Terminator
Conjugation, Genetic
DNA-Binding Proteins
Escherichia coli
Escherichia coli Proteins
F Factor
Gene Expression Regulation, Bacterial
Mutation
Promoter Regions, Genetic
Recombinant Fusion Proteins

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