Article
Clonal analysis of erythropoietin stimulated J2E cells reveals asynchrony during terminal differentiation.
Growth factors (Chur, Switzerland) - 1 Jan 1993
Busfield S J, Farr T J, Singh T, Sainsbury A J, Klinken S P
Abstract excerpt
J2E erythroid cells proliferate and differentiate in response to erythropoietin (epo), the red blood cell specific hormone. Using methylcellulose colony assays and suspension cultures we have demonstrated that nearly all the cells stimulated by epo synthesized haemoglobin. To achieve maximum production of haemoglobin J2E cells had to be treated with epo for only 6 h; hormone added subsequently did not enhance...
Topics
- Cell Division
- Cell Line
- Clone Cells
- Erythrocytes
- Erythropoiesis
- Erythropoietin
- Hemoglobins
- Phenotype