Mutation of the two carboxyl-terminal tyrosines in the insulin receptor results in enhanced activation of mitogen-activated protein kinase.

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Article

Mutation of the two carboxyl-terminal tyrosines in the insulin receptor results in enhanced activation of mitogen-activated protein kinase.

The Journal of biological chemistry - 8 Apr 1994

Pang L, Milarski K L, Ohmichi M, Takata Y, Olefsky J M, Saltiel A R

Abstract excerpt

Activation of mitogen-activated protein (MAP) kinase represents an important mechanism in hormonal regulation. To clarify the role of MAP kinase activation in insulin action, we compared the activation of the enzyme in Rat-1 cells transfected with wild-type (Hirc) and mutant insulin receptors in which the 2 carboxyl-terminal tyrosines were substituted with phenylalanine (Y/F2). Expression of the Y/F2 mutant...

Topics

Animals
Cells, Cultured
Enzyme Activation
Glutathione Transferase
Insulin Receptor Substrate Proteins
Mitogen-Activated Protein Kinase 1
Mutation
Phosphatidylinositol 3-Kinases
Phosphoproteins
Phosphorylation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Phosphotransferases (Alcohol Group Acceptor)

Protein Serine-Threonine Kinases

Protein-Tyrosine Kinases

Rats

Receptor, Insulin

Recombinant Fusion Proteins

Tyrosine

Source

Open in PubMed

Identifiers

PMID: 8144649

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8144649-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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